Factors That Affect Enzyme Reaction Rate

Lab Report F flirtors That Affect Enzymes Reaction come in Name of lab Effects of temperature, pH, Enzyme Concentration, and Substrate Concentration on Enzymatic Activity Introduction Enzymes ar the most important types of proteins, they act as catalysis (speed up chemical receptions). If enzymes didnt exist, biochemical reactions would act to slowly and they couldnt keep up with the metabolic functions. Enzymes have a three-dimensional structure that is rattling complex. This structure consists of one or more polypeptide chains, they form an active site, which is an area in which the substrate finally will fit.The four factors that affect the activity and reaction rate of an enzyme are temperature, pH, enzyme concentration and substrate concentration. enquiry Question/Hypothesis The hypothesis is that when the temperature is higher than 40? C, the enzyme catalysis will increase. That the enzyme activity with the pH force-out is that if it isnt at the optimum pH value on that point will be a list loss of the enzyme activity. As the change in enzyme concentration increases the activity rate will increase if there is a proportional amount of enzyme concentration and substrate.Variables Independent pH, enzyme concentration, substrate concentration and enzymatic activity. Dependent the reaction rate Control variable temperature and amount of substrates and enzymes added. Materials Phosphate Buffers Beaker Catechol Potato Juice Parafilm Test Tubes Procedure To view the effect of temperature 1. Three different canvas tobacco pipes where picked with 3mL of phosphate. 2. They were set in three different temperature settings. scratch tube was placed in an ice-water bath for ten minutes, the second in a room temperature until 21?C was r all(prenominal)ed, and the third tube was placed in a beaker of warm water. To claim the effect of pH 1. The pH values of pH 4, pH 6, pH 7, pH 8, and pH 10 was used to fill five separate block out tubes with 3 mL each. 2. T o each analyse tube, ten drops of catechol and spud juice were added, each tube was covered with Parafilm, and alter several times to mix the contents. After letting each tube stand for three to five minutes the tubes were inverted at one minute intervals. To study the effect of enzyme concentration 1.The steps include four test tubes that were labeled A, B, C, and D. 2. The contents added to tube A are 3mL plus 20 drops of pH 7 phosphate fender, but no potato juice. To tube B, 3 mL plus 15 drops of pH 7 phosphate soften and 15 drops of potato juice. The amount of pH 7 phosphate pilot light added to test tube C are 3 mL plus 10 drops of potato juice. To test tube D, 3 mL of pH 7 phosphate buffer and 20 drops of potato juice were added. 3. Then, each tube was covered in Parafilm and inverted several times to mix the contents.After doing so, 10 drops of catechol were added to each tube and covered with Parafilm and inverted several times to mix the contents. 4. After allowing e ach tube to stand for three to four minutes the tubes were mixed at one-minute intervals. To study the effects of substrate concentration 1. Eight test tubes that were involved. 2. These test tubes were labeled 1, 2, 4, 8, 16, 24, 32, and 48. Next, each test tube had a certain amount of phosphate buffer and catechol was added to each. 3.To test tube 1 5 mL plus 47 drops of pH 7 phosphate buffer and 1 drop of catechol test tube 2 5 mL plus 46 drops of pH 7 phosphate buffer and 3 drops of catechol test tube 4 5 mL plus 44 drops of pH 7 phosphate buffer and 4 drops of catechol test tube 8 5 mL plus 40 drops of pH 7 phosphate buffer and 8 drops of catechol test tube 16 5 mL plus 32 drops of pH 7 phosphate buffer and 16 drops of catechol test tube 24 5 mL plus 24 drops of pH 7 phosphate buffer and 24 drops of catechol test tube 32 5 Ml plus 16 drops of pH 7 phosphate buffer and 32 drops of catechol and test tube 48 5 mL of pH 7 phosphate buffer and 48 drops of catechol. 4. Then, each tub e was covered with Parafilm and inverted several times to mix the contents. Also, to each of the tubes, 30 drops of potato juice were added and covered with Parafilm and inverted several times to mix the contents. The test tubes set at room temperature for five minutes and were mixed at one minute intervals.